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human primary pulmonary artery vsmcs  (PromoCell)


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    Structured Review

    PromoCell human primary pulmonary artery vsmcs
    Morphology and comparative calcium responses to U46619 of blood outgrowth smooth muscle cells (BO-SMCs), vascular smooth muscle cells <t>(VSMCs),</t> <t>and</t> <t>fibroblasts</t> (HPFs). Phase contrast images of BO-SMCs, VSMCs, and fibroblasts were obtained using Zeiss Axio Observer WF1 or WF3 microscope at ×20 magnification (A) . Represented fluorescence images of cells stained with Flou-4 treated with U46619 (10 –6 M) (B) . Fluorescence intensity (fluorescence minus basal fluorescence taken at t = 0; F – F 0 ) tracings from n = 10 individual cells per donor imaged at 5–10 frames/s stained with Flou-4 treated with U46619 (10 –6 M) (C) . Pooled data showing mean ± SEM for the average intensity tracings obtained following U46619 (10 –6 M) treatment (BO-SMCs; n = 40 cells comprising 10 individual cells each from four separate donors, VSMCs and HPF; n = 30 cells comprising 10 individual cells from three separate donors) or untreated time controls (BO-SMCs; n = 30 cells comprising 10 individual cells each from three separate donors, VSMCs, and HPF; n = 20 cells comprising 10 individual cells from two separate donors) (D) . Scale bars represent 100 μm.
    Human Primary Pulmonary Artery Vsmcs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human primary pulmonary artery vsmcs/product/PromoCell
    Average 95 stars, based on 117 article reviews
    human primary pulmonary artery vsmcs - by Bioz Stars, 2026-05
    95/100 stars

    Images

    1) Product Images from "Activation and Contraction of Human “Vascular” Smooth Muscle Cells Grown From Circulating Blood Progenitors"

    Article Title: Activation and Contraction of Human “Vascular” Smooth Muscle Cells Grown From Circulating Blood Progenitors

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2021.681347

    Morphology and comparative calcium responses to U46619 of blood outgrowth smooth muscle cells (BO-SMCs), vascular smooth muscle cells (VSMCs), and fibroblasts (HPFs). Phase contrast images of BO-SMCs, VSMCs, and fibroblasts were obtained using Zeiss Axio Observer WF1 or WF3 microscope at ×20 magnification (A) . Represented fluorescence images of cells stained with Flou-4 treated with U46619 (10 –6 M) (B) . Fluorescence intensity (fluorescence minus basal fluorescence taken at t = 0; F – F 0 ) tracings from n = 10 individual cells per donor imaged at 5–10 frames/s stained with Flou-4 treated with U46619 (10 –6 M) (C) . Pooled data showing mean ± SEM for the average intensity tracings obtained following U46619 (10 –6 M) treatment (BO-SMCs; n = 40 cells comprising 10 individual cells each from four separate donors, VSMCs and HPF; n = 30 cells comprising 10 individual cells from three separate donors) or untreated time controls (BO-SMCs; n = 30 cells comprising 10 individual cells each from three separate donors, VSMCs, and HPF; n = 20 cells comprising 10 individual cells from two separate donors) (D) . Scale bars represent 100 μm.
    Figure Legend Snippet: Morphology and comparative calcium responses to U46619 of blood outgrowth smooth muscle cells (BO-SMCs), vascular smooth muscle cells (VSMCs), and fibroblasts (HPFs). Phase contrast images of BO-SMCs, VSMCs, and fibroblasts were obtained using Zeiss Axio Observer WF1 or WF3 microscope at ×20 magnification (A) . Represented fluorescence images of cells stained with Flou-4 treated with U46619 (10 –6 M) (B) . Fluorescence intensity (fluorescence minus basal fluorescence taken at t = 0; F – F 0 ) tracings from n = 10 individual cells per donor imaged at 5–10 frames/s stained with Flou-4 treated with U46619 (10 –6 M) (C) . Pooled data showing mean ± SEM for the average intensity tracings obtained following U46619 (10 –6 M) treatment (BO-SMCs; n = 40 cells comprising 10 individual cells each from four separate donors, VSMCs and HPF; n = 30 cells comprising 10 individual cells from three separate donors) or untreated time controls (BO-SMCs; n = 30 cells comprising 10 individual cells each from three separate donors, VSMCs, and HPF; n = 20 cells comprising 10 individual cells from two separate donors) (D) . Scale bars represent 100 μm.

    Techniques Used: Microscopy, Fluorescence, Staining

    Contraction responses to U46619 of blood outgrowth smooth muscle cells (BO-SMCs), vascular smooth muscle cells (VSMCs), and fibroblasts (HPFs). Representative images of contracting cells captured at 10 min after addition of cumulative concentrations of U46619 (1 × 10 –9 to 10 –6 M) (A) and pooled data (B) . Data in (B) are mean ± SEM; for BO-SMCs, n = 23 using cells from four separate donors; for VSMCs, n = 14 using cells from three separate donors; and for HPFs, n = 9 fields using cells from three separate donors. Filled symbols represent treated cells and open symbols represent untreated “time-control” (TC) cells. Statistical differences were tested using two-way ANOVA with a Sidak-multiple comparison post hoc test comparing individual U46619 concentration responses with cell-relevant time controls (* p < 0.05) or two-way ANOVA comparing the absence vs. presence of U46619 over time ( # p < 0.05). Data in panel 4 (bottom right hand graph) which include all cell types were tested using a two-way ANOVA ( # p < 0.05) comparing VSMCs or HPFs with BO-SMCs. Scale bars represent 100 μm.
    Figure Legend Snippet: Contraction responses to U46619 of blood outgrowth smooth muscle cells (BO-SMCs), vascular smooth muscle cells (VSMCs), and fibroblasts (HPFs). Representative images of contracting cells captured at 10 min after addition of cumulative concentrations of U46619 (1 × 10 –9 to 10 –6 M) (A) and pooled data (B) . Data in (B) are mean ± SEM; for BO-SMCs, n = 23 using cells from four separate donors; for VSMCs, n = 14 using cells from three separate donors; and for HPFs, n = 9 fields using cells from three separate donors. Filled symbols represent treated cells and open symbols represent untreated “time-control” (TC) cells. Statistical differences were tested using two-way ANOVA with a Sidak-multiple comparison post hoc test comparing individual U46619 concentration responses with cell-relevant time controls (* p < 0.05) or two-way ANOVA comparing the absence vs. presence of U46619 over time ( # p < 0.05). Data in panel 4 (bottom right hand graph) which include all cell types were tested using a two-way ANOVA ( # p < 0.05) comparing VSMCs or HPFs with BO-SMCs. Scale bars represent 100 μm.

    Techniques Used: Concentration Assay



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    human primary pulmonary artery vsmcs  (PromoCell)
    95
    PromoCell human primary pulmonary artery vsmcs
    Morphology and comparative calcium responses to U46619 of blood outgrowth smooth muscle cells (BO-SMCs), vascular smooth muscle cells <t>(VSMCs),</t> <t>and</t> <t>fibroblasts</t> (HPFs). Phase contrast images of BO-SMCs, VSMCs, and fibroblasts were obtained using Zeiss Axio Observer WF1 or WF3 microscope at ×20 magnification (A) . Represented fluorescence images of cells stained with Flou-4 treated with U46619 (10 –6 M) (B) . Fluorescence intensity (fluorescence minus basal fluorescence taken at t = 0; F – F 0 ) tracings from n = 10 individual cells per donor imaged at 5–10 frames/s stained with Flou-4 treated with U46619 (10 –6 M) (C) . Pooled data showing mean ± SEM for the average intensity tracings obtained following U46619 (10 –6 M) treatment (BO-SMCs; n = 40 cells comprising 10 individual cells each from four separate donors, VSMCs and HPF; n = 30 cells comprising 10 individual cells from three separate donors) or untreated time controls (BO-SMCs; n = 30 cells comprising 10 individual cells each from three separate donors, VSMCs, and HPF; n = 20 cells comprising 10 individual cells from two separate donors) (D) . Scale bars represent 100 μm.
    Human Primary Pulmonary Artery Vsmcs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human primary pulmonary artery vsmcs/product/PromoCell
    Average 95 stars, based on 1 article reviews
    human primary pulmonary artery vsmcs - by Bioz Stars, 2026-05
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Morphology and comparative calcium responses to U46619 of blood outgrowth smooth muscle cells (BO-SMCs), vascular smooth muscle cells (VSMCs), and fibroblasts (HPFs). Phase contrast images of BO-SMCs, VSMCs, and fibroblasts were obtained using Zeiss Axio Observer WF1 or WF3 microscope at ×20 magnification (A) . Represented fluorescence images of cells stained with Flou-4 treated with U46619 (10 –6 M) (B) . Fluorescence intensity (fluorescence minus basal fluorescence taken at t = 0; F – F 0 ) tracings from n = 10 individual cells per donor imaged at 5–10 frames/s stained with Flou-4 treated with U46619 (10 –6 M) (C) . Pooled data showing mean ± SEM for the average intensity tracings obtained following U46619 (10 –6 M) treatment (BO-SMCs; n = 40 cells comprising 10 individual cells each from four separate donors, VSMCs and HPF; n = 30 cells comprising 10 individual cells from three separate donors) or untreated time controls (BO-SMCs; n = 30 cells comprising 10 individual cells each from three separate donors, VSMCs, and HPF; n = 20 cells comprising 10 individual cells from two separate donors) (D) . Scale bars represent 100 μm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Activation and Contraction of Human “Vascular” Smooth Muscle Cells Grown From Circulating Blood Progenitors

    doi: 10.3389/fcell.2021.681347

    Figure Lengend Snippet: Morphology and comparative calcium responses to U46619 of blood outgrowth smooth muscle cells (BO-SMCs), vascular smooth muscle cells (VSMCs), and fibroblasts (HPFs). Phase contrast images of BO-SMCs, VSMCs, and fibroblasts were obtained using Zeiss Axio Observer WF1 or WF3 microscope at ×20 magnification (A) . Represented fluorescence images of cells stained with Flou-4 treated with U46619 (10 –6 M) (B) . Fluorescence intensity (fluorescence minus basal fluorescence taken at t = 0; F – F 0 ) tracings from n = 10 individual cells per donor imaged at 5–10 frames/s stained with Flou-4 treated with U46619 (10 –6 M) (C) . Pooled data showing mean ± SEM for the average intensity tracings obtained following U46619 (10 –6 M) treatment (BO-SMCs; n = 40 cells comprising 10 individual cells each from four separate donors, VSMCs and HPF; n = 30 cells comprising 10 individual cells from three separate donors) or untreated time controls (BO-SMCs; n = 30 cells comprising 10 individual cells each from three separate donors, VSMCs, and HPF; n = 20 cells comprising 10 individual cells from two separate donors) (D) . Scale bars represent 100 μm.

    Article Snippet: Human primary pulmonary artery VSMCs and pulmonary fibroblasts were obtained from PromoCell (Germany) and cultured in SMC and fibroblast growth media (5% FBS) respectively, following suppliers’ protocols (PromoCell, Heidelberg, Germany).

    Techniques: Microscopy, Fluorescence, Staining

    Contraction responses to U46619 of blood outgrowth smooth muscle cells (BO-SMCs), vascular smooth muscle cells (VSMCs), and fibroblasts (HPFs). Representative images of contracting cells captured at 10 min after addition of cumulative concentrations of U46619 (1 × 10 –9 to 10 –6 M) (A) and pooled data (B) . Data in (B) are mean ± SEM; for BO-SMCs, n = 23 using cells from four separate donors; for VSMCs, n = 14 using cells from three separate donors; and for HPFs, n = 9 fields using cells from three separate donors. Filled symbols represent treated cells and open symbols represent untreated “time-control” (TC) cells. Statistical differences were tested using two-way ANOVA with a Sidak-multiple comparison post hoc test comparing individual U46619 concentration responses with cell-relevant time controls (* p < 0.05) or two-way ANOVA comparing the absence vs. presence of U46619 over time ( # p < 0.05). Data in panel 4 (bottom right hand graph) which include all cell types were tested using a two-way ANOVA ( # p < 0.05) comparing VSMCs or HPFs with BO-SMCs. Scale bars represent 100 μm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Activation and Contraction of Human “Vascular” Smooth Muscle Cells Grown From Circulating Blood Progenitors

    doi: 10.3389/fcell.2021.681347

    Figure Lengend Snippet: Contraction responses to U46619 of blood outgrowth smooth muscle cells (BO-SMCs), vascular smooth muscle cells (VSMCs), and fibroblasts (HPFs). Representative images of contracting cells captured at 10 min after addition of cumulative concentrations of U46619 (1 × 10 –9 to 10 –6 M) (A) and pooled data (B) . Data in (B) are mean ± SEM; for BO-SMCs, n = 23 using cells from four separate donors; for VSMCs, n = 14 using cells from three separate donors; and for HPFs, n = 9 fields using cells from three separate donors. Filled symbols represent treated cells and open symbols represent untreated “time-control” (TC) cells. Statistical differences were tested using two-way ANOVA with a Sidak-multiple comparison post hoc test comparing individual U46619 concentration responses with cell-relevant time controls (* p < 0.05) or two-way ANOVA comparing the absence vs. presence of U46619 over time ( # p < 0.05). Data in panel 4 (bottom right hand graph) which include all cell types were tested using a two-way ANOVA ( # p < 0.05) comparing VSMCs or HPFs with BO-SMCs. Scale bars represent 100 μm.

    Article Snippet: Human primary pulmonary artery VSMCs and pulmonary fibroblasts were obtained from PromoCell (Germany) and cultured in SMC and fibroblast growth media (5% FBS) respectively, following suppliers’ protocols (PromoCell, Heidelberg, Germany).

    Techniques: Concentration Assay